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vectashield fluorescent mounting medium with dapi  (Vector Laboratories)


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    Structured Review

    Vector Laboratories vectashield fluorescent mounting medium with dapi
    Vectashield Fluorescent Mounting Medium With Dapi, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 98/100, based on 22048 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antifade+mounting+medium+for+fluorescence/VECTASHIELD+Antifade+Mounting+Medium+with+DAPI/10__1002_slash_pgr2__70054-63-4-10
    Average 98 stars, based on 22048 article reviews
    vectashield fluorescent mounting medium with dapi - by Bioz Stars, 2026-10
    98/100 stars

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    Related Articles

    Fluorescence:

    Article Title: TDP1 suppresses chromosomal translocations and cell death induced by abortive TOP1 activity during gene transcription.
    Article Snippet: .. Finally, cells were counterstained with DAPI (Sigma, D9542) and mounted in antifade mounting medium for fluorescence (Vectashield, Vector Labs, H-1000). .. Primary antibodies: anti-γH2AX (Millipore, 05-636) 1:1000 and anti-53BP1 (Novus Biologicals, NB100904) 1:2500.

    Article Title: Over-expression of V1A receptors in PVN modulates autonomic cardiovascular control.
    Article Snippet: After incubation in biotinylated anti-mouse IgG raised in horse (1:500, Vector Laboratories Ltd, Peterborough, UK, catalogue number: BA 2001) for one hour, sections were transferred in wells containing secondary donkey anti-goat CF594 antibody (1:500, Sigma-Aldrich Co. Ltd., Poole, Dorset, UK) and streptavidin conjugated Alexa Fluor 647 (1:500, Invitrogen Ltd, Paisley UK) diluted in PBS containing 0.3% (v/v) Triton X-100 for one hour. .. Following further rinses (3 x 10 min), sections were mounted onto slides in 0.5% gelatin, air-dried and cover slipped with antifade mounting medium for fluorescence (VectashieldTM, Vector Laboratories Ltd., Peterborough, UK). .. Images were observed using Zeiss Axioskop 20 fluorescent microscope.

    Article Title: TDP1 suppresses chromosomal translocations and cell death induced by abortive TOP1 activity during gene transcription
    Article Snippet: .. Finally, cells were counterstained with DAPI (Sigma, D9542) and mounted in antifade mounting medium for fluorescence (Vectashield, Vector Labs, H-1000). .. Primary antibodies: anti-γH2AX (Millipore, 05-636) 1:1000 and anti-53BP1 (Novus Biologicals, NB100-904) 1:2500.

    Article Title: SCAN1 mutant TDP1 blocks the repair of DSB induced by TOP1 activity during gene transcription and promotes genome reorganisations and cell death in quiescent cells
    Article Snippet: .. Finally, cells were counterstained with DAPI (Sigma, D9542) and mounted in antifade mounting medium for fluorescence (Vectashield, Vector Labs, H-1000). .. Primary antibodies: 53BP1 (Novus Biologicals, NB100-904) 1:2500, PAR (Millipore, MABE1016) 1:1000.

    Article Title: Death of retinal neurons in streptozotocin-induced diabetic mice.
    Article Snippet: RESULTS.. Morphometric analysis of retinal cross sections of mice that had been diabetic 14 weeks showed 20% to 25% fewer cells in the ganglion cell layer compared with agematched control mice.. There was a modest, but significant, decrease in the thickness of the whole retina and the inner and outer nuclear layers in mice that had been diabetic for 10 weeks.

    Article Title: Stripe rust resistance gene Wtk1 (Yr 15) and use thereof
    Article Snippet: .. Then, a solution of WGA conjugated to fluorophore Alexa 488 (L4895-2MG; Sigma-Aldrich) was placed on the leaf surfaces and stained for 24 h at 4° C. After washing with ddH2O, the stained leaf tissues were gently placed on microscope slides, immersed with antifade mounting medium for fluorescence (Vectashield, Vector Laboratories), covered with a cover glass, sealed with rubber cement, and stored at 4° C. in the dark. .. Fluorescent microscopy was carried out on an inverted fluorescent microscope, Leica DMi8 (Leica Microsystems, Wetzlar, Germany) fitted with a filter cube for the FITC excitation range (EX: 460-500, DC: 505, EM: 512-542) and a FLUO regime to observe the WGA-stained fungal structures.

    other:

    Article Title: H263A and SCAN1/H493R mutant TDP1 block TOP1-induced double-strand break repair during gene transcription in quiescent cells and promote cell death.
    Article Snippet: For sgRNA-mediated stable depletion of PNKP or TDP2, cells were infected with lentiviral particles generated using the vector #52961 (AddGene) and selected with 20ug/ml puromycin for 24–48 h. Pooled cells were tested for the loss of PNKP or TDP2 expression by western blotting.

    Article Title: H263A and SCAN1/H493R mutant TDP1 block TOP1-induced double-strand break repair during gene transcription in quiescent cells and promote cell death
    Article Snippet: hTERT RPE-1 cells (originally purchased from ATCC, CRL-4000) were propagated in DMEM/F12 medium supplemented with 10% fetal bovine serum (FBS) and with 1% penicillin and streptomycin.

    In Situ:

    Article Title: Death of retinal neurons in streptozotocin-induced diabetic mice.
    Article Snippet: RESULTS.. Morphometric analysis of retinal cross sections of mice that had been diabetic 14 weeks showed 20% to 25% fewer cells in the ganglion cell layer compared with agematched control mice.. There was a modest, but significant, decrease in the thickness of the whole retina and the inner and outer nuclear layers in mice that had been diabetic for 10 weeks.

    Whole Genome Amplification:

    Article Title: Stripe rust resistance gene Wtk1 (Yr 15) and use thereof
    Article Snippet: .. Then, a solution of WGA conjugated to fluorophore Alexa 488 (L4895-2MG; Sigma-Aldrich) was placed on the leaf surfaces and stained for 24 h at 4° C. After washing with ddH2O, the stained leaf tissues were gently placed on microscope slides, immersed with antifade mounting medium for fluorescence (Vectashield, Vector Laboratories), covered with a cover glass, sealed with rubber cement, and stored at 4° C. in the dark. .. Fluorescent microscopy was carried out on an inverted fluorescent microscope, Leica DMi8 (Leica Microsystems, Wetzlar, Germany) fitted with a filter cube for the FITC excitation range (EX: 460-500, DC: 505, EM: 512-542) and a FLUO regime to observe the WGA-stained fungal structures.

    Staining:

    Article Title: Stripe rust resistance gene Wtk1 (Yr 15) and use thereof
    Article Snippet: .. Then, a solution of WGA conjugated to fluorophore Alexa 488 (L4895-2MG; Sigma-Aldrich) was placed on the leaf surfaces and stained for 24 h at 4° C. After washing with ddH2O, the stained leaf tissues were gently placed on microscope slides, immersed with antifade mounting medium for fluorescence (Vectashield, Vector Laboratories), covered with a cover glass, sealed with rubber cement, and stored at 4° C. in the dark. .. Fluorescent microscopy was carried out on an inverted fluorescent microscope, Leica DMi8 (Leica Microsystems, Wetzlar, Germany) fitted with a filter cube for the FITC excitation range (EX: 460-500, DC: 505, EM: 512-542) and a FLUO regime to observe the WGA-stained fungal structures.

    Microscopy:

    Article Title: Stripe rust resistance gene Wtk1 (Yr 15) and use thereof
    Article Snippet: .. Then, a solution of WGA conjugated to fluorophore Alexa 488 (L4895-2MG; Sigma-Aldrich) was placed on the leaf surfaces and stained for 24 h at 4° C. After washing with ddH2O, the stained leaf tissues were gently placed on microscope slides, immersed with antifade mounting medium for fluorescence (Vectashield, Vector Laboratories), covered with a cover glass, sealed with rubber cement, and stored at 4° C. in the dark. .. Fluorescent microscopy was carried out on an inverted fluorescent microscope, Leica DMi8 (Leica Microsystems, Wetzlar, Germany) fitted with a filter cube for the FITC excitation range (EX: 460-500, DC: 505, EM: 512-542) and a FLUO regime to observe the WGA-stained fungal structures.



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